Research Assistant II

Position SummaryJoin our flow core team and provide high-quality flow cytometry and cell sorting services to Boston Children's investigators and the wider Boston research community. This is a full-time position with full Boston Children's Hospital benefits and a 2-year minimum commitment.Visit the Flow Cytometry webpage at Flow Cytometry | Boston Children's ResearchKey ResponsibilitiesPerform fluorescence-activated cell sorting (FACS), including aseptic sorting of human and mouse samples, and monitor sort purity and recoveryPerform daily quality control, laser alignment, and calibration (e.g., with standardized beads), plus maintenance and troubleshooting, on our high-speed cell sorters and benchtop analyzersAdvise investigators on experimental design, including multicolor panel design, compensation, and controls (e.g., FMO, viability dye)Analyze flow cytometry data (e.g., FlowJo) and assess data quality, and help investigators with gating strategy and presentation of resultsTrain new researchers and staff with instrument operation and good flow cytometry practice, and provide ongoing supportHandle billing, invoicing, and day-to-day lab operationsStay current in the field by attending vendor and technical training, participating in journal club, and giving presentations on flow cytometry methods and new technologies.Minimum QualificationsEducation: Bachelor's degree in biological or physical sciences (or related field) plus 2+ years of laboratory and flow cytometry experience in a clinical, industry, or academic setting, or a master degree in a related fieldExperience: ​​​​​​​Hands-on experience with multicolor flow cytometry; cell sorting experience preferredWorking knowledge of biosafety practices for handling human-derived samples (BSL-2)Ability to work independently, with strong problem-solving, attention to detail, and written and verbal communicationProficiency with PC and Mac platforms and Microsoft OfficeComfortably working in a diverse, multicultural research environment and is a team playerAbility to safely lift 50 lbs. (with or without accommodation)